The aim of this study was to asses the effect of Eucayptus camaldulensis on the invertebrate colonization, comparing the leaf litter from this exotic species with native ones: Miconia chartacea, Protium brasiliensis e Crotum sp (from Rain Forest and Cerrado/Savannah). The experiments were performed in three Brazilian streams: one at Rain Forest (Zé Adão) and two (Cipó and Taboões) at Cerrado/Savannah region. Each kind of leaf litter was air-dried, weighed about 3g, allocated inside bags of 10mm mesh size and placed into streams; four replicates were used for each treatment. Invertebrates were identified to family and had its density, taxonomic richness and biomass evaluated. There were no differences on invertebrate density and taxonomic richness between exotic and the native leaf litters (ANOVAone-way, p>0.05). However, the biomass of invertebrates colonizing E. camaldulensis was higher than in M. chartacea in Cipó stream and lower in Taboões stream (ANOVAOne-way, F= 13 and 4; p= 0.001 and 0.05), but we did not found difference between leaves for the Zé Adão stream (ANOVAone-way, p>0,05). Despite the differences on breakdown rate, there was no evidence of any negative effect of E. camaldulense on the invertebrate colonization, as stated in several papers.