To examine the influences of Acid mine drainage (AMD) on stream macroinvertebrate assemblages, we compared the species composition, density, Shannon-Wiener diversity index (H') and individuals of benthic macroinvertebrate from 8 sites.  The results showed that the diversity index H' and species richness significantly decreased in AMD-impacted sites where only 28 species were collected, and chironomids were most abundant group in these sites, however, a total number of 85 taxa were collected from the four reference sites. With the increase of distance to pollute source of AMD, pH of the downstream increased gradually, the abundance of macroinvertebrate was also increased, which suggests that purification capability of streams was recovered gradually at downstream.