Headwater streams are dominated by the detrital foodweb.  The extent of secondary production through this pathway is determined by both the quality (i.e. nutrient content) and the quantity of detritus available.  Urban streams are typically highly modified systems with drastically altered hydrology and geomorphology compared to more undisturbed streams.  In this study standing stocks of benthic organic matter (BOM) were determined along an urbanization gradient in the Louisville Metro area to determine how BOM standing crops vary with increasing levels of watershed urbanization.  Six streams were selected within the Louisville Metro area based on total watershed impervious surface area (0-47%).  Five benthic organic matter samples were collected monthly from March 2006 to February 2007 to determine standing stocks of coarse and fine benthic organic matter.  Coarse benthic organic matter (CBOM) was higher in the less urbanized streams, while fine benthic organic matter (FBOM) was higher in the more modified streams.  Lower CBOM in urbanized stream could be attributed to the reduced allochthonous inputs and organic matter retention.  Further work to determine retention and input of allochthonous organic matter will help to establish the possible mechanisms resulting in this variation along the urbanization gradient.