Management of stream systems and development of bioassessment standards are important for sustainable natural resources.  Macroinvertebrates and water quality data were collected June-August 2006 for evaluation of biological impact from wastewater effluent in southwest Ohio.  Quantitative macroinvertebrate samples were taken using a Hess sampler at ten sites.  Six samples were collected upstream and downstream of the outfall at each site and between 0.75-1.5 km downstream to assess recovery.   Four ecosystem attributes were determined: P/R, CPOM/FPOM, TFPOM/BFPOM and substrate channel stability.  Seven sites showed greater substrate stability and a higher level of suspended fine particulate organic matter downstream when compared to upstream; recovery sites were intermediate at some sites.  For example ratios at one site are P/R 0.151, 6.17, 0.506; CPOM/FPOM 0.0, 0.25, 0.011; TFPOM/BFPOM 0.039, 0.211, 0.138; and channel stability 0.196, 0.879, 0.711 with an increase from upstream to downstream and intermediate recovery values, respectively.  At this site the change is large enough to warrant a change of classification in channel stability.  In all cases with increases of autotrophy to heterotrophy ratios, the increase was in the downstream sites.  In general, downstream sites showed an increase in attribute ratios with intermediate recovery values for some sites.