Wetland biomonitoring has received attention in recent decades although stream monitoring has received far more interest. However, the environmental importance of ponds has been rarely studied. This study evaluated the richness and diversity of lentic aquatic macroinvertebrates to develop a biotic matrix for assessing pond environmental health. During spring 2006 and 2007, 10 fishless ponds, 5 accessed by cattle and 5 inaccessible to cattle, were sampled using macroinvertebrate funnel-traps. In 2006, 5574 individuals in 74 taxa were collected and in 2007, 3616 individuals in 83 taxa were collected. Evenness of sites were closely associated over both years and varied from moderate to high (0.56-0.88). Jaccard’s similarity was low and not significant in most comparisons with the exception of 7 of 45 in 2006 and 2 in 2007 which were significantly lower (p<0.05). Both cattle and non-cattle ponds had low Shannon values (2006 cattle pond, H´= 1.800±0.01 vs. non-cattle ponds H´= 1.992±0.001; 2007 cattle pond, H´=2.250±0.02 vs. non-cattle H´= 2.492±0.001). Although not significantly different, Shannon values higher in non-cattle ponds. Preliminary analysis of richness in some groups such as Hemiptera, Coleoptera, and aquatic mites may serve to identify impacts in these systems.