The reactivity of dissolved organic matter (DOM), a mixture of terrestrial and aquatic derived organic carbon, provides a source of reduced carbon for heterotrophic processes and releases nutrients upon mineralization.  Here we examine DOM reactivity across a multiple stream orders within agriculturally dominated streams in eastern Nebraska. Samples are collected monthly within six stream reaches ranging from 1^st through 6^th order in order to quantify temporal changes related to seasonal and hydrologic controls. Specific measurements include quantifying DOM bioavailability, DOM component analysis, DOM characterization through fluorescence, approximating DOM aromaticity, and nitrogen speciation.  We hypothesize that bioavailability is controlled by in situ production and the flushing of shallow flowpaths through the wetter spring and during event-driven periods during summer and fall.  Initial results show that DOM bioavailability decreases through the stream network, suggesting that net mineralization is greater than in situ production.  Aromaticity of DOM is lowest during the summer months, congruent with higher rates of production and more microbial derived organic matter.  Nitrogen species concentrations also decrease through the stream network, with nitrate concentrations gradually increasing from the summer into late fall.  Organic nitrogen and ammonia concentrations peak in early fall before decreasing again into late fall.