New plant-incorporated protectants (PIPs) in genetically modified plants may present a risk to aquatic invertebrates.  However, no current method exists to perform screening level toxicity testing for PIPs within an aquatic medium.  In an effort to develop an artificial diet for aquatic invertebrates, we adapted a diet created for marine invertebrates to be used on trichopteran larvae (shredders).  An ideal artificial diet would carry specific PIPs distributed evenly within its matrix, elicit feeding from the target organism, and allow a quantification of feeding, while delivering adequate nutrition to the larvae.  Experiments were performed to determine the concentration of nutrients within the diet that induced the greatest feeding response from larvae.  Experiments done without larvae quantified changes in the mass of the diet after five days immersed in water, which allowed a more accurate comparison to be made between the final and initial masses of the diet.  Tannic acid was incorporated into the diet to determine whether differences could be measured in the mass of the diet eaten by larvae once it is made less digestible.  Once developed, this artificial diet will be a powerful tool for performing tier-one bioassays for assessment of risk of PIPs to non-target aquatic organisms.